Fig. 6: Integrated analysis of MYT1L deficiency across neurodevelopment.
From: MYT1L deficiency impairs excitatory neuron trajectory during cortical development
A UMAP projection showing integrated data of 412,132 nuclei from E14, P1, and P21 datasets. Left panel: nuclei colored by cell classes. Middle panel: nuclei colored by 54 distinct clusters. Right panel: nuclei colored by developmental stage. B Mean ± SEM proportions of major cell classes across developmental stages in WT (top; n = 17 animals) and Het (bottom; n = 13 animals). C Mean ± SEM proportions of excitatory neuron subtypes across developmental stages in WT (top; n = 17 animals) and Het (bottom; n = 13 animals). UL ExN: upper layer excitatory neurons, DL ExN: deep layer excitatory neurons, Im: immature. D Venn diagram showing the number of differentially expressed genes identified by pseudobulk analysis in deep layer excitatory neurons (DL ExN) associated with age, genotype, and age:genotype interaction. E Expression patterns of genes showing significant age:genotype interaction across developmental stages in WT (blue) and Het (red) animals. Each line represents a gene, with box plots showing the distribution of scaled gene expression values. Box plots show median (center line), interquartile range (box), and whiskers extending to 1.5 times the interquartile range.
