Fig. 2: Binding kinetics of wildtype and a09b08 ImmTAVs to HBV Env371-379 pHLA-E*01:03 complexes.
From: Viral sequence determines HLA-E-restricted T cell recognition of hepatitis B surface antigen
a Binding curves for wildtype ImmTAV and all three HBV Env371-379 pHLA-E*01:03 complexes. Binding was determined over a range of analyte concentrations from 37 nM to 37.6 µM. Insets: calculation of steady-state affinity, data were presented as mean values ± SD. b Binding kinetics for a09b08 ImmTAV and all three HBV Env371-379 pHLA-E*01:03 complexes. Graphs show the mean of the raw data (blue) and the 1:1 fit (dotted red line). For the binding kinetic graphs, the ImmTAV molecule was flown over the chip as the analyte, at concentrations ranging from 0.313 to 5 nM. Kinetic constants were determined using a 1:1 Langmuir model. c Summary of KD values and t1/2 values of the wild-type and a09b08 ImmTAV. All experiments were performed at 25 °C in triplicate. Data were presented as mean values ± SD. Source data are provided as a Source Data file.
