Fig. 5: Active Nr1h2 partners with coactivator proteins to regulate blastoid formation.
From: Nuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts
a A schematic diagram showing transient overexpression of Nr1h2-FLAG in ESC with T09 treatment, followed by pull-down mass spectrometry and ChIP-seq of FLAG-tagged Nr1h2. Created in BioRender.com. b Quantification of blastoid formation efficiency harvested at Day 5 of differentiation from ESC overexpressing EGFP-FLAG, Nr1h2-FLAG or ΔLBD-Nr1h2-FLAGunder T09 treatment. One-way ANOVA with Bonferroni correction for multiple comparisons. Data are represented as mean ± s.d.; n = 3 independent assays. c Volcano plot summarising the quantitative results of the proteins enriched significantly in Nr1h2-FLAG mass spectrometry data in ESC with T09 from three replicates. The specific proteins interacting with Nr1h2 in ESC with T09 were colored red (Two-sided t-test; p-value < 0.05 & FC > 2). d Quantification of the blastoid efficiency after individual siRNA treatment in EPSC-blastoid or NrESC-blastoid formation. Two-way ANOVA with Bonferroni correction for multiple comparisons. Data are represented as mean ± s.d.; n = 3 independent assays. Source data are provided as a Source Data file.
