Fig. 5: Prophylactic efficacies of 5B11 against RSV A2 and B18537 infections in cotton rat model.
a The schematic diagram for RSV prophylaxis assay. b RSV titers in lungs and noses in each group of cotton rats five days after exposure to 2 × 106 pfu of RSV A2. Five animals were used per group (n = 5). c RSV titers in lungs and noses in each group of cotton rats five days after exposure to 4.8 × 105 pfu of RSV B18537. Four to five animals were used per group (n = 4–5). Error bars represent mean values with SD. The dotted line means the limit of detection. Statistical differences between groups were analyzed by one-way ANOVA, followed by Tukey’s multiple comparisons post hoc test. d–m Lung histopathology was quantitatively analyzed by a pathology scoring system in animals prophylactically inoculated by PBS and RSV F antibodies. Histology scores of cotton rats infected with RSV strain A2 (d–h) and B18573 (i–m) were respectively shown for the prophylactic evaluation of 5B11 and 1129 at 1.5 mg/kg and 15 mg/kg. Blood vessels (perivasculitis; d and i), bronchus (peribronchitis; e and j), bronchioles (peribronchiolitis; f and k), alveolar lumen (alveolitis; g and l) and lung interstitium (interstitial pneumonitis; h and m) in lung tissues were scored ranging from 0 to 4 points, according to the number of layers of inflammatory cells. Error bars represent mean values with SD. Kruskal-Wallis test was used for statistical differences between groups and corrected for multiple comparisons using Dunn’s method. Source data are provided as a Source Data file.
