Fig. 6: SAV1 inactivation suppresses Hippo signaling, leading to YAP activation. | Nature Communications

Fig. 6: SAV1 inactivation suppresses Hippo signaling, leading to YAP activation.

From: Whole-exome sequencing reveals genomic landscape of intrahepatic cholangiocarcinoma and identifies SAV1 as a potential driver

Fig. 6

A Western blot showed the expression of p-YAP, YAP, and the YAP upstream kinases pMST1/2, MST1, pLATS1, and LATS1 in ICC cells upon alteration of SAV1 expression. B, C Immunofluorescence staining showing subcellular YAP localization in the indicated cells. The graphs depict the percentages of cells with exclusively nuclear (N) YAP and with both nuclear and cytoplasmic (N + C) YAP. No cells showed YAP that was localized exclusively in the cytoplasm, n = 3, errors are in ±SD. Scale bar: 20 μm. D Representative SAV1 and YAP staining in tumor tissues with wild-type or mutant SAV1. Scale bars = 50 μm. The graphs depict the correlation between YAP nuclear localization and SAV1 mutation in ICC specimens. The subcellular localization of YAP was scored from 1 to 5, with 1 representing exclusive nuclear localization and 5 representing exclusive cytoplasmic localization. Student’s t-test, two-sided. sh short hairpin RNA, WT wild-type, MT mutation-type. Source data are provided as a Source Data file.

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