Fig. 2: CD8 TILs are heterogenous and contain a subset of cytotoxic cells expressing NKG2A.
From: IL-12 drives the expression of the inhibitory receptor NKG2A on human tumor-reactive CD8 T cells
a Gating strategy of CD8 T cells (left) and CD8 T cell subsets (right) using CITE-seq antibodies. b Unsupervised clustering of CD8 T cells from TILs (left) and projection of the 3 CD8 subsets identified in (b) onto the UMAP plot (right) (n = 6 HNSCC patients). c Normalized expression of selected markers defining tumor CD8 T cell clusters. d Naïve/TCM and Activation/exhaustion scores for each cell cluster. e RNA velocity of CD8 T cells projected on the UMAP plot. Arrows indicate the putative differentiation direction. Arrow sizes indicate the magnitude of predicted directionality. f Highlight of the cells most confidently assigned to each initial and terminal state on the UMAP. g Diffusion map of cells colored by CD8 subsets and microstates. h Gene expression dynamics of differentially expressed genes ordered along the latent time inferred by RNA velocity for the lineage 7. i Smoothed gene expression trends in pseudotime for the lineage 7.
