Fig. 7: IL-12 plays a central role in the induction of NKG2A on CD8 T cells.

Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and (b) summary of NKG2A expression after 8 days of culture are shown (n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, α-IL-12p70 Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of (c) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) (n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) (n = 3). b, d, e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p-values were determined by one-way ANOVA with Tukey’s post hoc test (b, d, g).