Fig. 1: Concept and workflow of the SCPro platform.
From: Multimodal single cell-resolved spatial proteomics reveal pancreatic tumor heterogeneity
The SCPro platform integrates multiple modules. a Antibody-guided cell typing based on high-quality multiplexed imaging and automated LMD with single-cell resolution. b Flow cytometry-based cell typing. c Ultra-high-sensitivity proteomics platform combining with ion exchange-based protein aggregation capture sample preparation, low-flow chromatography, and high-sensitivity mass spectrometry data acquisition. d Decoding the pancreatic tumor microenvironment through spatial deconvolution. CAF cancer-associated fibroblast, iCAF inflammatory CAF, myCAF myofibroblastic CAF, apCAF antigen-presenting CAF, Treg regulatory T cell, FFPE formalin-fixed paraffin-embedded, mIHC multiplexed immunohistochemical, LMD laser microdissection, TME tumor microenvironment.
