Fig. 3: Multicolor cell imaging and quantitative detection of redox substances.
From: Redox-switchable multicolor luminescent polymers for theragnosis of osteoarthritis
a CLSM images of 4T1 cells and chondrocytes after 2 h of incubation with PCys (λex = 405, 488, and 561 nm). b Schematic illustration of PCys detecting H2O2 and GSH in cells (ER: endoplasmic reticulum). c CLSM images of H2O2-pretreated or untreated 4T1 cells incubated with PCys-Cy5 for 2 h. d H2O2 levels in 4T1 cells with or without H2O2 pretreatment detected by PCys or H2O2 assay kit. e CLSM images of GSH-pretreated or untreated 4T1 cells incubated with PCys-s-Cy5 for 2 h. f GSH levels in 4T1 cells with or without GSH pretreatment detected by PCys-s or GSH assay kit. The scale bars in a, c, and e are 50 μm. Data are presented as the mean with SD (n  =  3 independent cells) in (d, f). Statistical differences were analyzed by a one-way ANOVA with Tukey’s multiple comparisons test. ns means no significance. Each experiment was repeated three times independently with similar results.
