Fig. 1: Knockout of Fam172a in the neurons of the hypothalamic Arc protects mice against HFD induced metabolic dysregulation.
From: Histone lactylation mediated by Fam172a in POMC neurons regulates energy balance
A Adult C57 BL/6 mice were fed a chow diet or HFD (4 or 8 weeks), and western blot analysis of Fam172a in Arc of hypothalamus was performed. n = 3 mice per group. B Expression of Fam172a in Arc of hypothalamus. Brain section of adult C57 BL/6 mice under a chow diet or HFD 8 weeks were immunostained for Fam172a (red). n = 5 mice per group. Cell nuclei were counterstained with DAPI (blue). Arc, arcuate nucleus; 3 V, third ventricle. Scale bars, 100 μm. C Expression of Fam172a in Arc of hypothalamus. Brain section of adult C57 BL/6 mice was coimmunostained for Fam172a (red) and Neu N (green). n = 4 mice. Cell nuclei were counterstained with DAPI (blue). 3 V, third ventricle. Scale bars, 100 μm. D Schematic diagram of virus injection in mice. Created in BioRender. Chen, Z. (2024) https://BioRender.com/j97w406. E AAV-Syn-GFP and AAC-Syn-Cre were injected into the Arc nucleus of adult Fam172aloxP/loxP mice. Immunofluorescence staining for Fam172a (red) and GFP (green) was then performed on brain sections at 4 weeks post-surgery. n = 4 mice per group. Cell nuclei were counterstained with DAPI (blue). 3 V, third ventricle. Scale bars, 100 μm. F AAV viruses were injected into the Arc of adult male Fam172aloxP/loxP mice fed a HFD. Body weight was then assessed. n = 8 mice per group. G–I AAV viruses were injected into the Arc of adult male Fam172aloxP/loxP mice fed a HFD. Representative DEXA images (G), fat mass (H) and lean mass (I) were then assessed. n = 8 (AAV-Syn-GFP) or 7 (AAV-Syn-Cre) mice per group. J, K Glucose tolerance test (GTT, J) and the area under the curve (AUC) of the GTT (K) of mice. n = 10 mice per group. L, M Oxygen consumption (VO2, L) and energy expenditure (EE, M) of the mice. lbm, lean body mass; Dark, dark cycle; Light, light cycle. n = 6 (AAV-Syn-GFP) or 5 (AAV-Syn-Cre) mice per group. Data are presented as mean ± SEM. two-tailed Student’s t-test (H, I, K), two-way ANOVA with Bonferroni’s post hoc test (F, J, L, M). Source data are provided as a Source Data file.
