Fig. 3: Activation of Fam172a in POMC neurons leads to an obesity-like phenotype.
From: Histone lactylation mediated by Fam172a in POMC neurons regulates energy balance
A AAV-GFP or AAV-Fam172a viruses were injected into the Arc of male adult POMC-Cre mice. Immunofluorescence staining for Fam172a (red) was then performed on brain sections. When Cre recombinase is present, infection with the AAV-GFP or AAV-Fam172a virus can lead to GFP expression. n = 4 mice per group. Cell nuclei were counterstained with DAPI (blue). 3 V, third ventricle. Scale bars, 100 μm. B AAV viruses were injected into the Arc of male adult POMC-Cre mice fed a normal chow. Body weight was then assessed. n = 7 (AAV-GFP) or 6 (AAV-Fam12a) mice per group. C–E AAV viruses were injected into the Arc of male adult POMC-Cre mice fed a normal chow. Representative DEXA images (C), fat mass (D) and lean mass (E) were then assessed. n = 8 (AAV-GFP) or 7 (AAV-Fam12a) mice per group. F The weight of three adipose tissues, such as BAT, sWAT and eWAT. n = 8 (AAV-GFP) or 7 (AAV-Fam12a) mice per group. G Representative H&E staining images of mouse adipose tissues. Scale bars, 100 μm. H, I GTT (H) and the AUC of GTT (I) of the mice. n = 7 mice per group. (J, K) ITT (J) and the AUC of ITT (K) of the mice. n = 7 mice per group. (L) Average food intake of the mice was assessed. n = 7 mice per group. (M, N) Oxygen consumption (VO2, M) and energy expenditure (EE, N) of the mice. lbm, lean body mass; Dark, dark cycle; Light, light cycle. n = 6 mice per group. Data are presented as mean ± SEM. two-tailed Student’s t-test (D, E, F, I, K, L), two-way ANOVA with Bonferroni’s post hoc test (B, H, J, M, N). Source data are provided as a Source Data file.
