Fig. 2: Inverse relationship between MEcPP levels and biofilm formation.
From: An evolutionarily conserved metabolite inhibits biofilm formation in Escherichia coli K-12
a, b Visualization of biofilms produced by CRISPRi strains (a), and MG1655 cells statically grown at room temperature in media with increasing concentrations of benzyl viologen (b), using crystal violet staining. c, d Analysis of relative biofilm mass calculated by the ratio between the OD570nm of dissolved crystal violet-stained biofilm and the OD600nm of planktonic cells at the time of staining (c) and bacterial growth measured at OD600nm (d). The results are derived from three biological replicates per genotype or treatment, and each biological replicate includes at least two technical replicates. Bars represent the mean value, and error bars show the standard deviations. Statistical tests used include Brown-Forsythe and Welch ANOVA and two-sided Dunnett’s multiple comparisons tests (CRISPRi strains) and RM one-way ANOVA tests with the Geisser-Greenhouse correction and two-sided Dunnett’s multiple comparisons tests (BV treatment). Source data are provided in a Source Data file.
