Fig. 5: MEcPP derepresses fimE transcription.
From: An evolutionarily conserved metabolite inhibits biofilm formation in Escherichia coli K-12
a, b Relative expression levels of fimE assessed in CRISPRi strains and ispg-2 fimE::Tn5 revertant (a), as well as in MG1655 cells grown in increasing concentrations of benzyl viologen (b). Bars represent the mean value, and error bars show the standard deviations. Statistical analyses used are Brown-Forsythe and Welch ANOVA and two-sided Dunnett’s multiple comparisons tests. The data are obtained from at least three biological replicates per genotype or treatment, with each biological replicate comprising two technical replicates. c, d Polyacrylamide gel visualization of the relative abundance of the ON and OFF phases in the fimA promoter in CRISPRi strains, the ispg-2 fimE::Tn5 revertant, and the fimE overexpressor in the revertant (ispg-2 fimE::Tn5 pTrc-fimE-sfGFP) (d), as well as in MG1655 strains grown in increasing concentrations of benzyl viologen (d). HinfI restriction digestion of the amplified fimA promoter generates differential band patterns, visualizing the ON (520 bp + 124 bp) and OFF (405 bp and 239 bp) phases of the promoter. Consistent results in (c and d) were confirmed in 2 additional experiments. Source data are provided in a Source Data file.
