Fig. 3: In vivo biodistribution and trapping of lysoTRAP.
From: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
a Schematic for the administration of Cyanine 7 (Cy7)-labeled lysoTRAP via pulmonary inhalation or intravenous (i.v.) injection and corresponding in vivo real-time fluorescence images of mice. b Corresponding signal profiles of lysoTRAP in a and corresponding area under the curve (AUC) calculation, showing the superior bioavailability of lysoTRAP in lungs via pulmonary inhalation. c Mean fluorescence intensity (MFI) statistics of the major organs dissected from C57BL/6 mice 9 h after the administration, and corresponding proportion of the lysoTRAP accumulation in lungs. d 3D reconstructed light-sheet microscopic image of a lung dissected from a mouse 24 h after pulmonary inhalation of Cyanine 5 (Cy5)-labeled lysoTRAP. The tracheas were labeled with streptavidin-fluorescein isothiocyanate (FITC). e H&E images of lung tissue sections after pulmonary inhalation of lysoTRAP or PBS. f Analysis of IFN-γ level in the supernatant of lung homogenate after pulmonary inhalation of lysoTRAP or PBS. g The evaluation of lung respiratory function after pulmonary inhalation of lysoTRAP or PBS, assessed as the tidal volume (the volume of air inhaled or exhaled per breath) (the blue background presents the normal range.) h Schematic for the detection of in vivo trapping SARS-CoV-2 pseudotyped virions by lysoTRAP in C57BL/6 mice. i Representative CLSM images of pseudotyped virion distribution in the lungs at 24 h of post-administration and corresponding quantitative analysis of fluorescence intensity (FI) alongside the white dotted line. j WB analysis of viral protein components (P55 and P24) in the lungs, showing the efficient protein degradation by h-lysoTRAP. k q-PCR analysis of viral RNA levels (GFP) in lungs. The data in b, c, f, g, and k represent the mean ± S.D. (n = 3 biologically independent mice). Statistical significance was calculated using two-tailed unpaired t-test in b and c, and two-tailed one-way ANOVA with multiple comparison test in k. Source data were provided in the Source data file.
