Fig. 4: In vivo clearance of pseudotyped and authentic SARS-CoV-2 by lysoTRAP.
From: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
a Schematic for the detection of in vivo clearance of pseudotyped virions by lysoTRAP in the hACE2 C57BL/6 mouse model. b Ex vivo imaging of pseudotyped virion (wild-type) GFP expression (inserted at the top of panel) and corresponding quantitative analysis of GFP fluorescence signals in the lungs dissected from hACE2 C57BL/6 mice after viral inoculation and administration with different treatments. c Representative fluorescence images of pseudotyped virion (wild-type) GFP expression in the lung slides and corresponding quantification analysis of the GFP signals. d, e Similar data as that presented in b,c following infection with the Omicron variant of pseudotyped virions. f Schematic for the detection of in vivo clearance of authentic SARS-CoV-2 by lysoTRAP in the hamster model. g q-PCR analysis of wild-type SARS-CoV-2 RNA copies in lungs at 3 d. h Representative immunofluorescent images of S expression in the lung tissues at 3 d. i Representative hematoxylin and eosin (H&E) images of the lung tissues at 3 d. The lungs in the PBS group showed abundant infection symptoms (alveolar septal thickening and inflammatory cell infiltration), while those symptoms were almost disappeared in lysoTRAP group. j Analysis of inflammatory cytokine (interleukin 6 (IL-6) and tumor necrosis factor (TNF)) mRNA levels in the supernatant of lung homogenate at 3 d via q-PCR detection. Actin was used as the housekeeping gene, and relative gene expression was normalized to the PBS group. k–n Similar data as that presented in g–j following infection with Omicron variant. The data in b, c, d, and e, represent the mean ± S.D. (n = 3 biologically independent mice). The data in g, j, k, and n represent the mean ± S.D. (n = 5 biologically independent hamsters). Statistical significance was calculated using two-tailed one-way ANOVA with multiple comparison test in b, c, d, and e, and two-tailed unpaired t-test in g, j, k, and n, respectively. Source data were provided in the Source data file.
