Fig. 1: Acute stress reduces muscle strength and induces behavior and cardiac abnormalities in Rbm24 S181A KI mice.

a, b Epinephrine decreased average and maximum grip strength in S181A KI mice. WT and A/A mice were injected intraperitoneally with epinephrine (E, 5 mg/kg), and then the average (a) and maximum (b) grip strength were recorded at 0, 30, or 120 min (n = 6), two-way analysis of variance ANOVA with Bonferroni post hoc correction for multiple comparisons. c TMT reduced open field activity of A/A mice. The total distance traveled (m) within 3 min was measured in the open field test before and after TMT exposure. Typical locomotion tracks in open-field tests were shown at the left, and quantification of the distance traveled in the total area was shown at the right (n = 6), two-way ANOVA with Bonferroni post hoc correction for multiple comparisons. d Detection of AF by ECG recordings of mice 1 day (D) or 3 days after E injection (1 mg/kg, once daily) as compared with mice before E injection. e E + SI led to the death of A/A mice. WT and A/A mice were injected with a single dose of E (2 mg/kg) intraperitoneally and subjected to social isolation (SI). The survival rate of mice was recorded (n = 10), log-rank Mantel-Cox test. f, g Echocardiographic analysis of left ventricular ejection fraction (LVEF) (f) and left ventricular fractional shortening (LVFS) (g) at baseline, 21 days of GH (group housing) post E, 21 days of SI post-S (saline), and 21 days of SI post E (E, n = 6; SI, n = 6; E + SI, n = 7), two-way ANOVA with Bonferroni post hoc correction for multiple comparisons. Data were shown as mean ± SD (a, b, c, f, g) from six to seven independent experiments.