Fig. 6: Stress induces S181 phosphorylation of RBM24 in the heart.

a, b Mice were pretreated with lithium (4 mmol/kg) or saline for 6 h, followed by E (a) or NE (b) (5 mg/kg) injection. Heart tissues were collected for WB analysis (n = 3). c The experiments were done as in (a) except that after lithium pretreatment, mice were exposed to 5,5-dihydro-2.4. 5-trimethylthiazoline (TMT) or distilled water (DW) for 10 min (n = 3). d Mice under social isolation (SI) showed fewer entries and spent less time in the open field center compared to group housing (GH) mice. Locomotion tracks (left) and quantification of center time, center distance, and total distance traveled (right) were shown (n = 6), two-tailed unpaired Student’s t-test, Data were shown as mean ± SD from six independent experiments. e Mice underwent SI or GH, and received intraperitoneally lithium (1 mmol/kg) or saline every 3 days for 30 days. Heart tissues were collected for WB analysis (n = 3). f Mice received AAV9-Scramble or AAV9-ShGSK3β (1 × 10¹² viral genomes, 100 μl). After 4 weeks, followed SI or GH. Heart tissues were analyzed by WB (n = 3). g H9C2 cells were transfected with eIF4E2 or scrambled siRNA for 72 h, followed by WB (n = 3). h. H9C2 cells were treated with Tat-e2-I (5, 10, 20 μM) or scrambled e2-I (20 μM) for 24 h and analyzed by WB (n = 3). i Mice were injected with TANNylated-e2-I (TA-e2-I) or TA-e2-S (0.5 mM peptide, [TA]/[peptide] ratio of 5), and heart tissues were collected 6 h later for WB analysis (n = 3). j RBM24 interacted with eIF4E2 but not with the eIF4E2(Δ1–15) mutant. GST-eIF4E2, GST-eIF4E2(Δ1–15), or GST bound to GST-agarose beads were incubated with His-RBM24, and the elution was analyzed by WB (n = 3). k The purified GST-eIF4E2 was incubated with different concentrations of e2R-I, and incubated with purified His-RBM24, followed by WB (n = 3). l H9C2 cells were treated with different concentrations of Tat-e2R-I (5, 10, 20 μM) or scrambled e2R-I (Scr, 20 μM) for 24 h and analyzed by WB (n = 3). m Mice were intravenously injected with TANNylated-e2R-I (TA-e2R-I) or TA-e2R-S. 6 h later, heart tissues were collected and analyzed by WB (n = 3).