Fig. 2: Functional characterization of R-mAb-bound AMPARs.
From: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors
a Representative mEPSCs traces from a dissociated hippocampal neuron before (pre) and after (5 min post) acute application of A1 scFv-Halo (scale bar is 15 pA/1 s). mEPSC frequencies and amplitudes, paired from the same cells pre- and post-A1 scFv-Halo application are shown below (n = 7 cells from 2 cultures, two-tailed Paired t-tests: frequency n.s. p = 0.4247, amplitude n.s. p = 0.8433). b Same as (a) but with application of A2 Fab-Halo (scale bar is 15 pA/1 s) (n = 7 cells from 2 cultures, two-tailed Paired t-tests: frequency n.s. p = 0.4523, amplitude n.s. p = 0.6656). c Experiment timeline (top) and representative images of cultured hippocampal neurons (bottom) treated with A1 or A2 R-mAbs pre-conjugated with JF635i or JF635i dye only (control) for 30 min. White arrowheads denote the targeted cell. Scale bars are 10 µm. d Representative mEPSCs traces from control neurons and neurons labeled with A1 scFv-Halo (scale bar is 15 pA/1 s). mEPSC frequency and amplitude are shown below (control n = 8 neurons, A1 scFv-Halo n = 13 neurons from 2 experiments, two-tailed Student’s t-tests: frequency n.s. p = 0.8651, amplitude n.s. p = 0.7421). e Same as (d) but with A2 Fab-Halo (JF635i) (scale bar is 15 pA/1 s) (control n = 12 neurons, A2 Fab-Halo n = 16 neurons, two-tailed Student’s t-tests: frequency n.s. p = 0.8503, amplitude n.s. p = 0.3848). f Same as (d) but with A2 scFv-Halo (JF635i) (control n = 9 neurons, A2 scFv-Halo n = 9 neurons, two-tailed Student’s t-tests: frequency n.s. p = 0.5337, amplitude n.s. p = 0.1371). Data are presented as mean values +/- SEM. n.s. not significant. Source data are provided as a Source Data file.
