Fig. 5: Functional characterization of i-mAb AMPAR labeling reagents.

a mEPSC traces from neurons expressing A1 scFv-Halo and controls, incubated with either vehicle or 2 µM tetrodotoxin/100 µM APV for 24 hr to induce homeostatic scaling (scale bar is 15 pA/1 s). b mEPSC frequency (left) and amplitude (right) from control neurons or neurons expressing A1 scFv-Halo 24 h following vehicle or APV/TTX treatment (control vehicle n = 10 neurons, control APV/TTX n = 10 neurons, A1 scFv-Halo vehicle n = 11 neurons, A1 scFv-Halo APV/TTX n = 11 neurons all from 2 cultures, Tukey’s multiple comparisons test, frequency control vehicle vs. control APV/TTX n.s. p = 0.9632, A1 scFv-Halo vehicle vs. A1 scFv-Halo APV/TTX n.s. p = 0.9988, control vehicle vs. A1 scFv-Halo vehicle n.s. p = 0.9916; amplitude control vehicle vs. control APV/TTX ****p < 0.0001, A1 scFv-Halo vehicle vs. A1 scFv-Halo APV/TTX ****p < 0.0001, control vehicle vs. A1 scFv-Halo vehicle n.s. p = 0.9045). c Hippocampal slices from mice expressing either A1 scFv-Halo-AAV or GFP-AAV (control) for 2 weeks were prepared for electrophysiological LTP recordings and IHC. d Example image of a CA1 principal neuron filled with biocytin from the recording pipette (stained with streptavidin, red) to confirm A1 scFv-Halo expression (JF585, cyan) (scale bar is 10 µm). e Plot of EPSC amplitudes (normalized to baseline) comparing LTP in CA1 pyramidal neurons expressing A1 scFv-Halo compared to control (infected with GFP-AAV) (control n = 6 neurons from 6 slices, A1 scFv-Halo n = 10 neurons from 10 slices). Representative EPSC traces pre- and 30 min post-LTP induction are shown to the right. Scale bars are 100 pA/200 ms. f Plot of normalized EPSC amplitudes 30 min post-LTP stimulus comparing control and A1 scFv-Halo expressing neurons (control n = 6 neurons from 6 slices, A1 scFv-Halo n = 10 neurons from 10 slices, two-tailed Student’s t-test, n.s. p = 0.8626). Data are presented as mean values +/- SEM. n.s. not significant. Source data are provided as a Source Data file.