Fig. 2: Linked-site recombination substrates.
From: Direct observation of subunit rotation during DNA strand exchange by serine recombinases
A Locations of the FRET donor (Alexa 488 or Atto 532) and acceptor (Cy5) positions in the linked-site Sin substrate SUL25. B Locations of the FRET donor (Alexa 488 or Cy3B) and acceptor (Cy5) in the linked-site Tn3 substrate UL25. C Potential recombination pathways for linked-site substrates. The dsDNA recombination sites are shown in grey and yellow and are joined by a flexible ssDNA linker (grey line). Resolvase subunits are shown as purple and blue circles and the biotin label attached to the 5′-end of the substrate is shown as a smaller magenta oval. Inversion (upper pathway) can be tracked by following distinct FRET changes at each stage. Deletion (lower pathway) is predicted to proceed with only small FRET changes.
