Fig. 1: The physical arrangement of DNA for the largest inversion and the distribution of nucleotide variation across all inversions.
a Inv_24: Each exon of IM767 from the interval of 1 to 7 mb of Chromosome 6 was mapped to IM664 (y-axis): blue = forward alignments (+), red = reverse alignments (−). The inversion contains two distinct sections of negative alignment separated by a positive alignment. b The average (and distribution) of nucleotide diversity (π) is reported ancestral-ancestral (πAA), ancestral-derived (πAD), and derived-derived (πDD) comparisons for genes within inversion interiors (left), inside the breakpoints (middle), and immediately outside breakpoints (right). The dotted line is the mean π for genes across the colinear genome. Means were compared by ANOVA and the bands are 95% confidence intervals. Samples sizes for interior genes are n = 51, 51, and 26 for AA, AD, and DD, respectively. The corresponding n are 54, 53, 28 for inside breakpoints and n = 54, 53, 27 for outside breakpoints. The diversity statistics are reported as a function of (c) the frequency of the derived orientation within IM and (d) the physical size of the inversion. In c, no estimate is reported for πDD where q = 0.1, because interval variation cannot be calculated unless there are at least two lines carrying the orientation. Points have been ‘jittered’ slightly for q to reveal distinct observations. Source data are provided as a Source Data file.
