Fig. 7: Sequence features of PC DNA. | Nature Communications

Fig. 7: Sequence features of PC DNA.

From: Structural basis for C. elegans pairing center DNA binding specificity by the ZIM/HIM-8 family proteins

Fig. 7

a Mutations in the TG sub-site (changing TG to CA) within the X, V, and II/III chromosome PC motifs result in the complete disruption of their binding to HIM-8 ZF1-2-CTD, ZIM-2 ZF1-2-CTD, and ZIM-1 ZF1-2-CTD, respectively. Nucleotides marked in red are mutating nucleotides. b Mutation of A7G8 to GC in the X chromosome PC motif leads to reduced binding to HIM-8 ZF1-2-CTD. Nucleotides marked in red are mutating nucleotides. c Mutation of A7G8 to GC in the X chromosome PC motif leads to enhanced binding to ZIM-2 ZF1-2-CTD. These results indicate that the sequence between the TTGG and TG sub-sites determines the binding specificity of HIM-8 and ZIM-2. Nucleotides marked in red are mutating nucleotides. d Reducing the number of base pairs between the TTGG and TG sub-sites to 3, 2, and 1 results in a significant reduction in their binding affinities to HIM-8 ZF1-2-CTD. Nucleotides labeled in red deletion line represent the deleting nucleotides.

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