Fig. 8: Dynamic stem cell behaviors regulated by remote tuning of # ligand inter-cluster edges is driven by the adhesion-relevant molecular machinery. | Nature Communications

Fig. 8: Dynamic stem cell behaviors regulated by remote tuning of # ligand inter-cluster edges is driven by the adhesion-relevant molecular machinery.

From: Modularity-based mathematical modeling of ligand inter-nanocluster connectivity for unraveling reversible stem cell regulation

Fig. 8

a Immunostained fluorescent images of YAP co-stained with F-actin and nuclei of adherent stem cells after 48 h of culturing in stem cell growth medium or RUNX2 co-stained with F-actin and nuclei after 72 h of culturing in stem cell differentiation medium on materials displaying remotely tunable # ligand inter-cluster edges. In each case, the medium was supplemented with ML9 (a myosin II-inhibitor), Swinholide A (Swinh. A; an actin polymerization-inhibitor), or Y27632 (a ROCK-inhibitor) or without any of the inhibitors (the control). b Computation of nuclear/cytoplasmic intensity ratio of YAP (n = 6 cells; ***p < 0.001) and RUNX2 (n = 6 cells; *p < 0.05; ***p < 0.001) expression in adherent stem cells. Scale bar: 50 µm (confocal microscopy). Data are shown as the means ± standard errors. Statistical analysis was performed using one-way ANOVA along with the Tukey–Kramer post hoc test. N.S. denotes no statistically significant difference. Source data are provided as a Source Data file.

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