Fig. 5: Hotspots of transcription-associated positive superhelical stress.
From: Osmotic disruption of chromatin induces Topoisomerase 2 activity at sites of transcriptional stress
A–D 10 bp binned CC-seq-v2 maps of physiological Top2ccs in G1-arrested S. cerevisiae, over 50 kb range at the indicated loci in untreated cells (top) and at the indicated minutes after osmotic shock with 0.6 M sorbitol. Red and blue traces indicate Top2-linked 5′ DNA termini on the top and bottom strands, respectively. In each track, the pale red and blue curves are the same data smoothed with a sliding 1 kb Hann window. Coloured arrows in the bottom panel indicate positions and orientation of specific genomic elements, with stranded RNA-seq data plotted below. E–H) Average (pileup) of CC-seq-v2 signals around long gene transcription termination sites (TTSs) (E), convergent gene arrays (F), Ty elements (G), and strong GapR-seq peaks (H), respectively at the indicated timepoints following osmotic shock. Data are smoothed with a sliding 2 kb Hann window. The peak time points of osmotic shock are indicated by brackets.
