Fig. 4: msh6-G233A elevates mutation rates in neutral background and in natural isolates from clade A.

a Each point shows the calculated mutation rate from a single independent fluctuation test using 12 replicate cultures. Each of the three points for the MFIG001 msh6-G233A variant is a separate independent transformant. Error bars show 95% confidence intervals. Fold change shows the median fold change in mutation rate from the parental MFIG001 strain. b Mean fitness of msh6-G233A relative to MFIG001 across five transfers, the cross bar shows the median (three independent transformants, to replicates each for a total of N = 6), the lower and upper hinges correspond to the first and third quartiles and the whiskers extend to 1.5*IQR. Box plots coloured by media type. The horizontal dashed line shows equal fitness. c Mutation rate of natural genotypes to olorofim. Each point shows the calculated mutation rate from a single independent fluctuation test using 12 replicate cultures. Error bars show 95% confidence intervals. Fold difference shows the median fold change in mutation rate of isolates with G233A allele from clade B isolates. Points coloured by clade, red clade A, blue clade B. Key below plot shows the presence of azole resistance mutation cyp51A TR₃₄/L98H in orange, TR₄₆/Y121F/T289A in blue, and the presence of msh6-G233A in grey. Source data are provided as a Source Data file.