Fig. 3: PD1+ TIGIT+ CD8+ exhausted T cells are enriched in the tumors from Nf1-OPG mice. | Nature Communications

Fig. 3: PD1+ TIGIT+ CD8+ exhausted T cells are enriched in the tumors from Nf1-OPG mice.

From: Human single cell RNA-sequencing reveals a targetable CD8+ exhausted T cell population that maintains mouse low-grade glioma growth

Fig. 3

A Schematic representation of T cell circulation from the blood through the dural meninges (dura), optic nerve (ON), and draining deep cervical lymph nodes (dCLN). Created in BioRender. Chatterjee, J. (2023) BioRender.com/f03c341. B t-SNE visualization of scRNAseq data from 12-week-old Nf1-OPG mice peripheral blood mononuclear cells (PBMCs), based on cell type; n = 10 pooled mice. C t-SNE visualization of scRNAseq data from 12-week-old Nf1-OPG PBMCs, color-coded by gene expression (Cd8a, Pdcd1, Tigit, Lag3). D t-SNE visualization of the T cell populations from 12-week-old Nf1-OPG mouse meningeal dura, color-coded by T cell type, n = 10 pooled mice. E Dot plot showing the genes used to identify CD8+ exhausted T cells in the 12-week-old Nf1-OPG mouse meningeal dura scRNAseq dataset. Representative gating strategy for flow cytometry of 12-week-old PMBC T cells (n = 9 mice) (F), meningeal dura (n = 9 mice) (G), optic nerve (n = 4, 3 pooled optic nerves per sample) (H), and deep cervical lymph nodes dura (n = 9 mice) (I). J Dot plot showing the genes used to identify CD8+ exhausted T cells in 12-week-old Nf1-OPG mouse optic nerves (corresponds to the dataset shown in Fig. 2; n = 10 pooled mice), meningeal dura (corresponds to the dataset shown in (D); n = 10 pooled mice), and PBMC (corresponds to the dataset shown in (B); n = 10 pooled mice) scRNAseq datasets. K Graph representing the percentages of CD8+/PD1+ and CD8+/TIGIT+ cells in the optic nerves (ON) and dura of 12-week-old Nf1-OPG mice (ON, n = 4, 3 pooled optic nerves per sample; dura, n = 9 mice). Data are represented as mean ± SD. A one-way ANOVA test was performed followed by a Dunnett’s post-test correction. Exact P values are indicated. L Graph comparing the percentages of CD8+/PD1+/TIGIT+ cells in the PBMCs, dura, optic nerve (ON), and dCLN, of 12-week-old Nf1-OPG mice (PBMCs, n = 8 mice; dura, n = 9 mice; ON, n = 4, 3 pooled optic nerves per sample; dCLN, n = 8 mice). Data are shown as mean ± SD. A one-way ANOVA test was performed followed by a Holm-Šídák post-test correction. Exact P values are included. M Representative gating strategy for flow cytometry of one representative human PA1 (n = 1; corresponds to PA1 sample in Fig. 1F).

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