Fig. 5: Deletion of FLRT2 affects the morphology of adherens junctions in vivo.
From: Vascular FLRT2 regulates venous-mediated angiogenic expansion and CNS barriergenesis
Vascular front of P5 retinas (a) and remodeling capillaries in cerebral cortex (c) from control and Flrt2iΔEC littermates stained for VE-cadherin antibody. Blood vessels visualized with IB4. Quantification of VE-cadherin activity in retina (b) and cerebral cortex (d) blood vessels. 15 × 15 μm regions of interest (ROIs) were blindly classified to a VE-cadherin activity category: low (smooth pattern), medium (irregular pattern), high (rough pattern). Example images of the three VE-cadherin activity categories in retina (b) and cortex (d) vessels are shown in correlation with the graph. n = 66 control and 56 mutant images from three litters (b), 78 control and 80 Flrt2 mutant images from two litters (d). Two-way ANOVA, p = 0.012 (low), 0.693 (medium), 0.999 (high) for b; 0.023 (low), 0.009 (medium) and 0.854 (high) for d. Scale bars: 20 μm (a), 15 μm (c). Data are shown as median (thick dashed line) and quartiles (thin dashed lines). *P > 0.05, **P < 0.01, ns = not significant.
