Fig. 5: Disrupting the SNX17-Retriever interaction impairs PM protein homeostasis.

Volcano plots summarizing TMT-based proteomic quantification of plasma membrane proteins, comparing WT to EV cells (a), and WT to DM cells (b). This experiment was performed once, with each group consisting of six biological replicates. Two-sided Student’s t test and Benjamini–Hochberg false discovery rate (FDR) correction were applied in data analyses. Colored dots (blue or green) denote proteins with FDR-corrected p value < 0.05; green dots depict hits that also had >2-fold change. Dot size is proportional to the position on the y axis (depicting statistical significance). c Heat map of PM protein abundance quantified by TMT-based proteomics after surface biotinylation and streptavidin purification, using indicated HeLa stable cell lines (shown in Fig. 4d). Only proteins whose abundance was significantly different between WT and EV lines (p value < 0.05) are displayed. Membrane proteins are denoted in brown font. Detailed proteomic data are provided in Supplementary Data 1 and deposited in MassIVE repository, as detailed in the data availability section.