Fig. 6: Disrupting the SNX17-Retriever interaction impairs PM protein trafficking and endosomal morphology.

Representative confocal images (a) and quantification of colocalization (b) derived from concurrent immunofluorescence staining for the cargo protein ITGA5 (green) and the endosomal marker FAM21 (red) in the indicated cell lines. c, d Similar to a, b but focusing on the cargo protein ITGB1 (green). Flow cytometry analysis of surface ITGB1 in indicated HeLa cell lines, showing scatter plots depicting gating (e), a representative histogram depicting ITGB1 surface staining (f), and mean fluorescence intensity compared to WT cells in the indicated HeLa cell lines (g). In g, aggregate data from 2 independent experiments are shown, with each dot representing a biological replicate. Representative confocal images (h) and quantification of the area of FAM21-positive endosomes (i) derived from immunofluorescence staining of FAM21 in the indicated HeLa stable cell lines. In all quantifications, each dot represents an individual cell, with number of cells in each group indicated above the graph. Mean and standard deviation are shown. One-way ANOVA with Dunnett’s correction was used. All imaging and FACS experiments were performed at least twice. Representative results are shown.