Fig. 3: DA neuron over-excitation during adolescence mediates long-lasting social deficits. | Nature Communications

Fig. 3: DA neuron over-excitation during adolescence mediates long-lasting social deficits.

From: Synaptotagmin-11 deficiency mediates schizophrenia-like behaviors in mice via dopamine over-transmission

Fig. 3

a Schematic showing the co-injection of TH-Cre and DIO-hM3D-mCherry/DIO-mCherry viruses into the VTA of juvenile (3 weeks) C57 mice. b–d Representative heat maps and statistics of the three-chamber social interaction test of juvenile (6–8 weeks) hM3D-expressing mice vs control mice following i.p. administration of CNO (0.5 mg/kg). e, f Representative heat maps and statistics of the social novelty test of juvenile hM3D-expressing mice vs control mice as described in b–d. g Statistics of sniffing time in the social approach test of juvenile hM3D-expressing mice vs control mice following i.p. administration of CNO. h Statistics of investigation frequency and investigation time in the home-cage social test of juvenile hM3D-expressing mice vs control mice following i.p. administration of CNO. i Schematic showing the co-injection of TH-Cre and DIO-hM3D-mCherry/DIO-mCherry viruses into the VTA of neonatal C57 mice (hM3D, P0) and the experimental procedure. j–l Representative heat maps and statistics of sniffing time and social index in the three-chamber social interaction test of juvenile repetitive CNO-treated (every second day during P7-P14) hM3D-expressing mice vs control mice as described in i. m, n Representative heat maps and statistics of sniffing time and total social time in the social novelty test of juvenile repetitive CNO-treated hM3D-expressing mice vs control mice. o Sniffing time of juvenile repetitive CNO-treated hM3Dq-expressing mice vs control mice in the social approach test with a caged novel mouse. p Investigation frequency and investigation time of juvenile repetitive CNO-treated hM3Dq-expressing mice vs control mice in the home-cage social test. Data are shown as box-and-whisker plots, with the median represented by the central line inside each box, the 25th and 75th percentiles represented by the edges of the box, and the whiskers extending to the most extreme data points. Ordinary two-way ANOVA followed by Bonferroni’s multiple comparisons for (c, e, k, m), two-tailed Mann-Whitney test for (d, f–h, l, n–p), *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significant difference. Source data are provided as a Source Data file.

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