Fig. 3: Structural differences between N559 and N559-M6. | Nature Communications

Fig. 3: Structural differences between N559 and N559-M6.

From: Evolutionary insights into the stereoselectivity of imine reductases based on ancestral sequence reconstruction

Fig. 3

a Differences in the overall protein conformation caused by the six historical mutations. The protein structure of N559 is shown on the left with the two monomers colored green and deep teal. Structural superposition of N559 and N559-M6 is shown on the right with N559-M6 colored pink. The shift in the Rossmann fold region is indicated by a black arrow. b Distribution of the six historical substitutions around the NADP+-binding site of N559-M6. Mutated residues in N559-M6 are shown in stick representation. c Sectional view of the substrate-binding pockets of N559 (left) and N559-M6 (right) accommodating NADP+.

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