Fig. 2: Polarity of Cdc42 and Tubulin in HSCs.
From: Quantitative determination of the spatial distribution of components in single cells with CellDetail
a Left: Confocal IF images (example from z-stacks) of Cdc42, Tubulin and DAPI (scale bar 5 µm). Right: Absolute value of Pn, with median as central mark, edges indicating 25th and 75th percentiles, whiskers extending to most extreme data points not considered outliers, outliers individually plotted. Median Cdc42: Pn,y = 1.0e-6, Pn,o = 0.7e-6, p = 2.3e-24, common language effect size (CLES) DCdc42 = 0.25, median Tubulin: Pn,y = 1.1e-6, Pn,o = 1.0e-6, p = 0.8, CLES DTubulin = 0.49, ny = 217, no = 372 b Left: Widefield IF images (example from z-stacks) of Cdc42, Tubulin and DAPI, scale bar 5 µm. Right: Absolute value of Pn, with median as central mark, edges indicating 25th and 75th percentiles, whiskers extending to most extreme data points not considered outliers, outliers individually plotted. Median Cdc42: Pn,y = 0.89e-6, Pn,o = 0.68e-6, p = 1e-12, CLES DCdc42 = 0.40, median Tubulin: Pn,y = 0.60e-6, Pn,o = 0.55e-6, p = 0.009, CLES DTubulin = 0.46, ny = 630, no = 1370. c Z-layers of individual cells (3D) and their Pn values of Cdc42 and Tubulin. d Percentage of cells over their Pn for young, old, and old HSCs treated with CASIN (nyoung = 92, nold = 98, noldCASIN = 96, median Pn,young = 0.81e-6, Pn,old = 0.64e-6, Pn,oldCASIN = 0.84e-6, pyoung,old = 0.0032, pyoung,oldCASIN = 8.0e-4, pyoung,oldCASIN = 0.9, CLES Dyoung,old = 0.38, Dold,old treated = 0.36) (a–d) all two-sided Wilcoxon-Ranksum test. e Cumulative percentage of HSCs in dependence on Pn value distribution (data from (d)). f Scheme for determination of dnucleus via |MR−| (green arrow) and the maximal diameter dmax (orange arrow). g Pearson correlation coefficients RPearson of the Pn of DAPI, Cdc42 and Tubulin vs. dnucleus, with p < 0.05 for all correlations shown (two-sided). pold,dnucleus,Cdc42 = 2.8e-129, pold,dnucleus,Tubulin = 0.0024, pold,dnucleus,DAPI = 7.3e-157; pyoung,dnucleus,Cdc42 = 1.0e-30, pyoung,dnucleus,Tubulin = 0.001, pyoung,dnucleus,DAPI = 2.0e-46. Source data are provided as a Source Data file.
