Fig. 3: The organization of the Septin network in HSCs changes upon aging.
From: Quantitative determination of the spatial distribution of components in single cells with CellDetail
a IF images (example from z-stacks) of a young or old HSC (scale bar 5 µm) of Septins 1,2,6,7,9,11. b Absolute value of Pn, with median as central mark, edges indicating 25th and 75th percentiles, whiskers extending to most extreme data points not considered outliers, outliers individually plotted. Common language effect size (CLES) DSept2 = 0.35, DSept6 = 0.36, DSept7 = 0.28, DSept9 = 0.30, DSept11 = 0.34; pSept1 = 0.27, pSept2 = 2.0e-12, pSept6 = 5.6e-11, pSept7 = 1.5e-24, pSept9 = 7.1e-22, pSept11 = 1.2e-13, ny = 330 and no = 440. c Distance d |between positive charge centers (box plots, median as central mark, edges indicating 25th and 75th percentiles, whiskers extending to most extreme data points not considered outliers, outliers individually plotted), ny = 330 and no = 440. CLES DSept6-Sept7 = 0.45, DSept7-Sept9 = 0.41, DSept7-Sept11 = 0.45. pSept6-Sept7 = 0.03, pSept7-Sept9 = 2.9e-5, pSept7-Sept11 = 0.03. d Graphical representations of examples of the distance d between charge centers. e Angle α between different positive charge centers of distinct Septins in young or old HSCs, median, ny = 330 and no = 440. CLES DSept6-Sept7 = 0.38, DSept6-Sept11 = 0.40, pSept6-Sept7 = 6.3e-9, pSept6-Sept11 = 1.4e-6. f Graphical representations of examples of the angle α between charge centers. g Pearson correlation coefficient values (RPearson,image, output parameter of CellDetail) of the intensity channels for distinct combinations of Septins (box plots, median as central mark, edges indicating 25th and 75th percentiles, whiskers extending to most extreme data points not considered outliers, outliers individually plotted), pSept2-Sept6 = 2.2e-4, pSept2-Sept9 = 0.02, pSept2-Sept11 = 0.02, pSept6-Sept9 = 0.01, pSept7-Sept9 = 7.2e-6, pSept9-Sept11 = 0.006, ny = 330 and no = 440. CLES for distributions with significant different medians are: D2-6 = 0.42, D2-9 = 0.45, D2-11 = 0.45, D6-9 = 0.45, D7-9 = 0.41, D9-11 = 0.44. b, c, e, g two-sided Wilcoxon Ranksum test. Source data are provided as a Source Data file.
