Fig. 4: The level of organization of the network of Septin proteins in aged HSCs can be restored to a youthful level by pharmacological attenuation of the activity of the small RhoGTPase Cdc42.
From: Quantitative determination of the spatial distribution of components in single cells with CellDetail
a IF images (examples) of one z-stack plane of old or old HSC treated with CASIN (scale bar 5 µm) of Cdc42 and Septins 6,7,9. b Pn of Cdc42 and Septins 6,7, 9 (box plots, with median as central mark, bottom and top edges of box indicating 25th and 75th percentiles and whiskers extending to most extreme data points not considered outliers, and outliers individually plotted). nold = 57, nold,CASIN = 60 HSCs the same old mice. Median, two-sided Wilcoxon-Ranksum test, pCdc42 = 0.01, pSept6 = 0.0036, pSept7 = 1.5e-4, pSept9 = 3.7e-4. Common language effect size (CLES): DCdc42 = 0.36, DSeptin6 = 0.34, DSeptin7 = 0.30, DSeptin9 = 0.31. c RSpearman of Pn of Cdc42 and Pn of Septins of old and old+CASIN HSCs. nold = 57, nold,CASIN = 60. pold,Cdc42Sept6 = 0.22, pold,Cdc42Sept7 = 0.19, pold,Cdc42Sept9 = 0.01, poldCASIN,Cdc42Sept6 = 0.048, poldCASIN,Cdc42Sept7 = 0.03, poldCASIN,Cdc42Sept9 = 0.03. Source data are provided as a Source Data file.
