Fig. 5: Polarity of Septins and the organization of the Septin network in human dermal fibroblasts is affected by senescence/aging.
From: Quantitative determination of the spatial distribution of components in single cells with CellDetail
a IF images (examples) of one z-stack plane of young and senescent human FF95 dermal fibroblasts for Septins 6,7,11 and Tubulin (scale bar 50 µm). b Absolute value of Pn, with median as central mark, edges indicating 25th and 75th percentiles, whiskers extending to most extreme data points not considered outliers, outliers individually plotted. Common language effect size (CLES): DSept6 = 0.39, DSept11 = 0.39, two-sided Wilcoxon-Ranksum test (pSept6 = 0.04, pSept7 = 0.96, pSept9 = 0.4, pSept11 = 0.05, pTubulin = 0.6), nCPD4.3 = 50, nCPD59.0 = 68. c Pearson correlation coefficients (RPearson) of paired-channel-based Pearson correlation coefficients (RPearson, output parameter of CellDetail) for distinct combinations of Septins and Tubulin of young and senescent human FF95 dermal fibroblasts (nCPD4.3 = 50, nCPD59.0 = 68) and right, their difference in correlation coefficients. Except for 7-Tub with 7-9 in CPD 59.0 FF95 cells, all correlations were significant (p < 0.05, two-sided; provided within Source Data file). Source data are provided as a Source Data file.
