Fig. 2: Preparing OCDC vaccine-induced neoantigen reactive T cells (NRT) is feasible. | Nature Communications

Fig. 2: Preparing OCDC vaccine-induced neoantigen reactive T cells (NRT) is feasible.

From: A dendritic cell vaccine for both vaccination and neoantigen-reactive T cell preparation for cancer immunotherapy in mice

Fig. 2

a Schematic diagram of the immunogenicity verification of the screened neoantigens. b Flow cytometric results of splenic lymphocytes from each group of mice after coculture with DCs loaded with immunogenic neoantigen peptides (n = 3 biologically independent samples). c Flow cytometry results of TILs from each group of mice after coculture with DCs loaded with immunogenic neoantigen peptides (n = 3 biologically independent samples). d Uniform manifold approximation and projection (UMAP) plots of flow-sorted CD3 + CD137+ single cells from NRTs. e Circle plot showing the differentially expressed gene (DEG) expression in each cell cluster. f Dot plots showing the expression of representative genes in each cell cluster. g Triangle diagram showing the results of enrichment analysis for each cell cluster. h Dot plots showing the differentially expressed TFs in each cell cluster. i Pseudotime trajectory analysis of the sorted CD137 + T-cell subclusters. The direction of the inferred pseudotime is indicated by the arrow. j Pseudotime trajectory analysis of the sorted CD137 + T-cell subsets. The proportions of clonal cells in different cell clusters are shown in the bar graphs in the upper part of the figure. The direction of the inferred pseudotime is indicated by the arrow. Source data are provided as a Source Data file.

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