Fig. 3: Antitumor effects of OCDC vaccination combined with NRT adoptive transfusion.

a Experimental design. b Schematic of the vaccine immunization and T-cell infusion time points. c Tumor growth curves of individual mice (n = 12 independent animals in each group). d Mean tumor volume (n = 12 independent animals in each group). e Tumor weight (n = 6 independent animals in each group). f Survival curves (n = 6 independent animals in each group). g Representative images showing the proportion of GFP + T cells in tumor tissues of each group (n = 3 biologically independent samples). h Statistical results of the proportion of GFP + T cells in tumor tissues of each group (n = 3 biologically independent samples). i Multi-fluorescence immunohistochemistry shows the GFP-positive T cells and GFP-negative T cells in OCDC + T group and OCDC + NRT group (n = 3–4 independent fields of view from two independent experiments; where the yellow arrows represent GFP-positive T cells, and the white arrows represent other T cells in the tumor microenvironment; scale bar, 20 µm). j Representative images showing the proportion of GFP + IFN-γ + T cells in tumor tissues of the OCDC + T group and OCDC + NRT group (n = 3 biologically independent samples). k Statistical results of the proportion of GFP + IFN-γ + T cells in tumor tissues of each group (n = 3 biologically independent samples). l Representative images showing the proportion of GFP-IFN-γ + T cells in tumor tissues of each group (n = 3 biologically independent samples). m Statistical results of the proportion of GFP-IFN-γ + T cells in tumor tissues of each group (n = 3 biologically independent samples). Data were expressed as the mean ± SD (d, e, h, k, and m) and Kaplan-Meier plot (f). Unpaired t test (d), Ordinary one-way ANOVA with Tukey’s multiple comparisons test (e, m) and Log-rank (Mantel-Cox) test (f) were used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Source data and P values are provided as a Source Data file.