Fig. 7: Neoantigen-specific TCR-T cells construction and functional validation. | Nature Communications

Fig. 7: Neoantigen-specific TCR-T cells construction and functional validation.

From: A dendritic cell vaccine for both vaccination and neoantigen-reactive T cell preparation for cancer immunotherapy in mice

Fig. 7

a Experimental design (Created in BioRender. Min, D. (2024) https://BioRender.com/n63q768). b Expression of neoantigen peptide-recognizing TCRs in CD4+ Neo TCR-T cells. c Expression of neoantigen peptide-recognizing TCRs in CD8+ Neo TCR-T cells. d Apoptotic effects of Neo TCR-T cells on tumor cells (n = 3 biologically independent samples). e Tumor cells survival rates measured by real-time cell analyzer (n = 3 biologically independent samples). f Expression of Neurl4 peptide-recognizing TCRs in CD4+ Neo TCR-T cells (n = 3 biologically independent samples). g Expression of Neurl4 peptide-recognizing TCRs in CD8+ Neo TCR-T cells (n = 3 biologically independent samples). h Expression of IFN-γ in CD4+ Neo TCR-T cells (n = 3 biologically independent samples). i Expression of IFN-γ in CD8+ Neo TCR-T cells (n = 3 biologically independent samples). Data were expressed as the mean ± SD (d–i). Two-way ANOVA (d, e) and unpaired t test (f, g, h, i) were used for statistical analysis, *p < 0.05, **p < 0.01, ***p < 0.00 l and ****p < 0.0001. Source data and P values are provided as a Source Data file.

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