Fig. 8: Detection the myeloid cells in the TME.

a UMAP plots of myeloid cells, colored by cell type. b Circle diagram showing the DEGs in each cell cluster, colored by cell type. c Proportions of each cell type in each group, colored by cell type. d Triangle plots showing the differences in DC functions in different groups, colored according to group. e Box plots showing the enrichment scores of the activated DC signatures across groups (NS: n = 125; DC: n = 174; OCDC: n = 136; OCDC + T: n = 36; OCDC + NRT: n = 123). f Circos plot of the cellular crosstalk between DCs and other cell types in the OCDC + NRT group relative to the remaining groups. The top 30 differentially expressed DC ligands are shown. Pink represents cellular crosstalk that is upregulated in the OCDC + NRT group relative to the remaining groups, and purple represents cellular crosstalk that is specific to the OCDC + NRT group relative to the remaining groups. g Representative multiplex fluorescence immunohistochemistry images show DCs in each group (n = 3-4 independent fields of view from two independent experiments; scale bar, 20 µm). h Representative images showing the proportion of M1-like macrophages and M2-like macrophages within the TME of each group. i Representative multiplex fluorescence immunohistochemistry images show M1-like macrophages and M2-like macrophages in each group (F4/80 + CD163- represents like macrophages, and F4/80 + CD163+ represents M2-like macrophages; n = 3–4 independent fields of view from two independent experiments; scale bar, 20 µm). The boxes showing the median (horizontal line), second to third quartiles (box), and Tukey-style whiskers (beyond the box) (e). Statistical analysis was performed by two-sided Wilcoxon rank-sum test (e), *p < 0.05, and ****p < 0.0001. Source data and P values are provided as a Source Data file.