Fig. 1: Polymer Screening for Xenogeneic-free Culture of ISCs3D-hIO. | Nature Communications

Fig. 1: Polymer Screening for Xenogeneic-free Culture of ISCs3D-hIO.

From: Xenogeneic-free culture of human intestinal stem cells on functional polymer-coated substrates for scalable, clinical-grade stem cell therapy

Fig. 1

a Schematic illustration of polymer synthesis in the vapor phase using the iCVD process and ISCs3D-hIO cultivation without xenogeneic ECM on polymers. b Chemical structures of 26 types of polymer candidates for the culture of ISCs3D-hIO. (X_Y denotes the copolymer composed of X and Y, and +p indicates a surface that has undergone postplasma treatment.) c Morphologies of ISCs3D-hIO cultured on a Matrigel-coated surface, various polymer-coated surfaces and tissue culture polystyrene (TCPS) (n = 2 sample/group). Optical microscope images of each culture surface were captured on Day 4. The scale bar indicates 500 µm. d Heatmap of polymers with surface energy, colony areas at Days 2 and 4, and growth rates.

Back to article page