Fig. 5: Formation of Intestinal Tissues on XF-DISC. | Nature Communications

Fig. 5: Formation of Intestinal Tissues on XF-DISC.

From: Xenogeneic-free culture of human intestinal stem cells on functional polymer-coated substrates for scalable, clinical-grade stem cell therapy

Fig. 5

a Workflow of intestinal tissue formation on XF-DISC. b External appearance of bare (left) and XF-DISC (right) inserts. Scale bar: 5 cm. c Surface views of 3D intestinal tissues subjected to ALI differentiation on a Matrigel-coated surface and XF-DISC for 0, 2, 4, 6, and 8 days at passage 17 (n = 3 samples/group). Scale bar: 500 µm. d Gene expression of markers of human small intestine and ALI differentiated intestinal tissues on a Matrigel-coated surface and XF-DISC for 0, 4 and 8 days at passage 6, as quantified by qRT‒PCR. Data represent the mean ± SD (n = 3 biological samples). Statistical significance between Matrigel D0 cells and other cells was assessed by one-way ANOVA followed by Tukey’s post hoc test. e Representative images showing immunostained sections of ALI differentiated intestinal tissues on a Matrigel-coated surface and XF-DISC for 0, 4 and 8 days at passage 6 (n = 3 samples/group). Scale bar: 100 µm. f Representative images showing histological sections of ALI differentiated intestinal tissues on a Matrigel-coated surface and XF-DISC for 0, 4 and 8 days at passage 6. Scale bar: 20 µm (n = 3 samples/group). g TEER values of ALI-differentiated intestinal tissues on a Matrigel-coated surface and XF-DISC for 2, 4, 6, and 8 days at passage 5. Data represent the mean ± SD (n = 3 biological samples), and a two-tailed t-test was applied to measure p values.

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