Fig. 6: Transplantation of ISCs3D-hIO Cultured on XF-DISC in EDTA-induced Intestinal Epithelial Injury. | Nature Communications

Fig. 6: Transplantation of ISCs3D-hIO Cultured on XF-DISC in EDTA-induced Intestinal Epithelial Injury.

From: Xenogeneic-free culture of human intestinal stem cells on functional polymer-coated substrates for scalable, clinical-grade stem cell therapy

Fig. 6

a Workflow of ISC3D-hIO transplantation in NOD/SCID mice. b Representative mouse colonoscopic observations over time. c Top, histopathology of the xenograft colon tissues based on H&E staining (n = 5 samples/group). Bottom, mucin staining of the xenograft colon tissues based on AB‒PAS staining (n = 2 samples/group). Scale bar: 500 µm. d Box and scatterplots of the crypt depth of fibrin-only (n = 246 crypts from three mice; 87, 80, and 79 crypts respectively) and ISCs3D-hIO on XF-DISC (n = 303 crypts from three mice; 89, 117, and 97 crypts respectively) treated xenograft colon tissues following H&E staining. The quartiles of the boxplot are mean ± SD, and a Welch’s unpaired t-test was applied to measure p value. e Human E-cadherin staining of xenograft tissues, indicating xenotransplanted ISCs3D-hIO cultured on XF-DISC (n = 2 from each group). Scale bar: 100 µm. f, Bright-field images of the main organs after xenotransplantation (n = 4).

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