Fig. 7: Transplantation of ISCs3D-hIO Cultured on XF-DISCs in DSS-induced Colitis. | Nature Communications

Fig. 7: Transplantation of ISCs3D-hIO Cultured on XF-DISCs in DSS-induced Colitis.

From: Xenogeneic-free culture of human intestinal stem cells on functional polymer-coated substrates for scalable, clinical-grade stem cell therapy

Fig. 7

a Workflow of ISC3D-hIO transplantation in NOD/SCID mice. b Representative mouse colonoscopic observations over time. c Top, histopathology of the xenograft colon tissues by H&E staining (n = 10 samples/group). Bottom, mucin staining of the xenograft colon tissues by AB‒PAS staining. Scale bar: 500 µm (n = 2 samples/group). d Box and scatterplots of the crypt depth of fibrin-only (n = 228 crypts from three mice; 76, 81, and 71 crypts respectively) and ISCs3D-hIO on XF-DISC (n = 714 crypts from six mice; 115, 131, 110, 118, 65, and 175 crypts respectively) treated xenograft colon tissues following H&E staining. The quartiles of the boxplot are mean ± SD, and a Welch’s unpaired t-test was applied to measure p-value. e Human E-cadherin staining of xenograft tissues, indicating xenotransplanted ISCs cultured on XF-DISC (n = 2 from each group). Scale bar: 100 µm.

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