Fig. 2: Physically associated and functionally implicated ibrutinib binding candidates.

a Network plot showing the CORUM complex composition of the sub-network of top NPARC hits and their associations according to the BioGRID interaction database. Nodes are plotted by size according to connectivity, and colored labels indicate membership in an enriched CORUM complex. b Functional proteoform group melting behavior for the HOPS complex, showing results flagged in the enrichment analysis input where at least one individual cell line NPARC result showed a shift meeting a significance threshold of p < 0.05, which include: VPS11_1, VPS11_2, VPS16_1, VPS16_2, VPS18_1, VPS18_2, VPS33A_1, and VPS33A_2. The melt curves for each condition represent n = 10 measurements each, each of the 10 temperature measurements performed in duplicates for each experimental condition and cell line, and are plotted with 95% confidence intervals of 4PL curve fits. Each point shows the mean fraction non-denatured and error bars show ±SD. The complex enrichment result was significant when evaluated using data from both cell lines, pAdj = 0.0392. c Functional proteoform group melting behavior for the WASH complex, showing flagged in the enrichment analysis input where at least one individual cell line NPARC result showed a shift meeting a significance threshold of p < 0.05, which include: WASHC2C_1, WASHC4_1, WASHC4_2, WASHC4_3, WASHC5_1, WASHC5_2, and WASHC5_3. The melt curves for each condition represent n = 10 measurements each, each of the 10 temperature measurements performed in duplicates for each experimental condition and cell line, and are plotted with 95% confidence intervals of 4PL curve fits. Each point shows the mean fraction non-denatured and error bars show ±SD. The complex enrichment result was significant when evaluated using data from both cell lines, pAdj = 0.0392, and in SW13, pAdj = 0.0348. Source data are provided as a Source Data file.