Fig. 5: DP2 antagonism or DP1 agonism enhances NK cell IFN-γ production and alters the phenotype of alveolar macrophages. | Nature Communications

Fig. 5: DP2 antagonism or DP1 agonism enhances NK cell IFN-γ production and alters the phenotype of alveolar macrophages.

From: Dual therapy with corticosteroid ablates the beneficial effect of DP2 antagonism in chronic experimental asthma

Fig. 5

Mice with CEA were inoculated with RV-1b and treated with a DP2 antagonist or a DP1 agonist daily starting from day 99. Mice were euthanized at 1, 3 and 7 dpi. A Number of IFN-γ expressing cells in the lungs at 7 dpi. B Number of IFN-γ expressing CD4+ T, CD8+ T and NK cells in the lungs at 7 dpi. C Number of TNF expressing cells in the lungs at 7 dpi. D Number of TNF expressing CD4+ T, alveolar macrophages (AMs) and conventional type-2 dendritic cells (cDC2s) in the lungs at 7 dpi. E Representative flow cytometry plots showing TNF expressing AMs in the lungs. F Number of total AMs in the lungs. G Representative flow cytometry plots showing CD86 and CD206 expression on AMs and the total number of CD206-expressing AMs and CD86-expressing AMs. Data are presented as mean ± SEM or box-and-whisker plots showing individual data points with the boxes representing quartiles and whiskers indicating the range and are pooled data from two independent experiments showing similar results (n = 4–8 mice per group). Statistical significance between different time points or different groups was determined using one-way ANOVA with Dunnett’s multiple comparison test. * denotes p < 0.05; ** denotes p < 0.01 and *** denotes p < 0.001 compared to vehicle group at 0 dpi. # denotes p < 0.05, ## denotes p < 0.01 and ### denotes p < 0.001 compared to RV-infected group at corresponding time point. Source data are provided as a Source Data file.

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