Fig. 3: Mina53-mediated H4R3me2a demethylation is important for proliferation and differentiation of neural stem/progenitor cells. | Nature Communications

Fig. 3: Mina53-mediated H4R3me2a demethylation is important for proliferation and differentiation of neural stem/progenitor cells.

From: Mina53 demethylates histone H4 arginine 3 asymmetric dimethylation to regulate neural stem/progenitor cell identity

Fig. 3

a Representative immunoblotting analysis of histone methylation marks and PRMT1 in proliferating (Proli) and differentiated (Differ) NSPCs. b Representative immunoblotting analysis of H4R3 methylation status and PRMT1 in proliferating NSPCs depleted of Mina53. c Representative images and quantification of BrdU immunofluorescence staining of NSPCs in the presence or absence of Mina53 depletion (n = 3, three different fields of view for each group). d Schematic depiction of mice crossing to generate Mina53 conditional knockout (cKO) mice. e Representative immunoblotting analysis of H4R3 methylation status in NSPCs isolated from control (Ctrl) and Mina53 cKO mice. f Representative images and quantification of Nestin-BrdU immunofluorescence staining of NSPCs isolated from control (Ctrl) and Mina53 cKO mice (n = 3, three mice for each group). The experiments related to (a, b, and e) have been performed in duplicate independently. For statistics in this figure, the two-tail unpaired Student’s-t test was utilized in (c) and (f), and data were shown as means ± SD. Source data are provided as a Source Data file.

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