Fig. 2: Single cell resolution of fibroblast and mural cell subsets in human SjS salivary glands.
From: Molecular and spatial analysis of tertiary lymphoid structures in Sjogren’s syndrome
a Uniform manifold approximation and projection (UMAP) dimensional reduction and sub-clustering of fibroblast and mural cell transcriptomic data in Sjogren’s syndrome (SjS) minor salivary glands (mSG). b Expression of current identifying and newly discovered identifying genes across the fibroblast and mural subsets. c Expression of gene cassettes used to spatially identify immunofibroblast and CCL21 CCL19 pericytes in salivary glands. d Multiplex immunofluorescence image illustrating immune cells and pericytes identified in minor salivary gland tissue (n = 2) probed with a 6-plex panels for CD138 (green), CD20 or TNC (yellow), Pan-Cytokeratin/CK or CD82 (orange), VE-cadherin (cyan), Podoplanin/PDPN (white), CD3 or CD146 (red) and DAPI or CD45 (blue), Scale bar = 20 µm. e Multiplex immunofluorescence image of CD146 (grey) pericytes with ccl21rna (green), ccl19rna (red) and DAPI (blue) in immune aggregate within human salivary glands of patients (n = 2) with Sjögren’s syndrome, Scale bar = 20 µm. f Representative flow cytometric identification of CD146+TNC+ CD36+CCL21+ pericyte cell population in Sjogren’s syndrome minor salivary glands. g Gene-set overrepresentation analysis (using GO and Kegg gene sets) of the fibroblast and mural populations, the top 5 enriched gene sets for each cell subset are shown.
