Fig. 5: Bulk RNA-sequencing of microdissected regions of minor salivary glands from Sjogren’s syndrome patients and control tonsil tissue.
From: Molecular and spatial analysis of tertiary lymphoid structures in Sjogren’s syndrome
a PCA plot of bulk-sequencing of microdissection samples. b Z-score mean expression of genes important to tertiary lymphoid organogenesis, in bulk-sequencing of microdissection data. c PCA plot of Nanostring Geomx protein expression data. d Z-score mean expression of proteins important to tertiary lymphoid organogenesis, in Nanostring Geomx data. e Cibersortx inferred cell subset proportions in the bulk sequencing samples using the single cell sequencing data as a reference atlas. Tonsil germinal center (GC) = 3, tertiary lymphoid structure germinal center (TLS GC) = 19, Segregated = 7, Non segregated = 4. Boxplots show the median, first and third quartiles, and whiskers extend to the largest value no further than 1.5 times the interquartile range.
