Table 1 Human flow cytometry antibody panel
From: Molecular and spatial analysis of tertiary lymphoid structures in Sjogren’s syndrome
Marker | Clone | Isotype | Conjugate | Concentration (µl) | Supplier |
|---|---|---|---|---|---|
CD36 | 5-271 | Mouse IgG2a, κ | BV421 | 1/100 | Biolegend |
TNC | 4C8MS | Mouse IgG1 | AF700 | 1/100 | Novus Biologicals |
CD146 | P1H12 | Mouse IgG2a, κ | BV786 | 1/100 | Biolegend |
CD34 | 581 | Mouse IgG1, κ | BV510 | 1/50 | Biolegend |
CD31 | WM59 | Rat IgG2a, κ | Alexa Fluor 488 | 1/200 | Biolegend |
EpCAM | 9C4 | Rat IgG2a, κ | Alexa Fluor 488 | 1/400 | Biolegend |
CD45 | HI30 | Rat / IgG2b, kappa | Alexa Fluor 488 | 1/300 | Biolegend |
CCL21 | AF366 | Polyclonal Goat IgG | Unconjugated | 1/100 | R&D Systems |
Anti-Goat 2° Antibody | N/A | Donkey / IgG | PE | 1/100 | Invitrogen |
