Table 2 Mouse flow cytometry antibody panel
From: Molecular and spatial analysis of tertiary lymphoid structures in Sjogren’s syndrome
Marker | Clone | Isotype | Conjugate | Concentration (µl) | Supplier |
|---|---|---|---|---|---|
CD34 | Ram34 | Rat / IgG2a, kappa | Alexa Fluor 700 | 1/100 | Invitrogen |
CD146 | ME-9F1 | Rat IgG2a | 1/100 | Biolegend | |
CD31 | 390 | Rat IgG2a, κ | BV421 | 1/200 | Biolegend |
EpCAM | G8.8 | Rat IgG2a, κ | BV605 | 1/400 | Biolegend |
CD45 | 30-F11 | Rat / IgG2b, kappa | BV711 | 1/300 | Biolegend |
CCL21 | AF457 | Polyclonal Goat IgG | Unconjugated | 1/50 | R&D Systems |
Anti-Goat 2°Antibody | N/A | Donkey / IgG | Alexa Fluor 647 | 1/100 | Invitrogen |
